PerfeCTa® qPCR SuperMixes, Quanta Biosciences

Unique buffer and stabilizers have been specifically optimized to deliver maximum PCR efficiency, sensitivity, and a robust fluorescent signal with TaqMan® or TaqMan® MGB probe chemistry. The enhanced specificity of this supermix suppresses cross-reactivity between homologous sequences, improving detection and discrimination in SNP applications. A key component of this supermix is AccuStart™ Taq DNA polymerase with monoclonal antibodies that bind to the polymerase and keep it inactive prior to the initial PCR denaturation step. Upon heat activation （two minutes at 95°C）, the antibodies denature irreversibly, releasing fully active, unmodified Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. 
 

Supermixes are available and optimized for all real-time PCR instrument platforms including those requiring normalization with ROX™ dyes. Supermixes are also available with uracil-N-glycosylase （UNG） to prevent amplification of carry-over contamination from previous dU-containing PCRs.